Natural Resource Ecology Laboratory

Protocols

One of the challenges faced by environmental enzyme researchers is the variety of enzymes assays that are available. Variation in our techniques makes it more difficult to compare data across studies and ecosystems. We’d like to use the RCN to establish first a repository of protocols and then hopefully establish standard protocols that will be applied by most researchers in the field.

If you have a protocol that you’d like to submit, please send it to enzymes@nrel.colostate.edu (subject: RCN-Protocol). Also, please see the “Experimental Notes” area for a repository of all the things that went wrong & what we’ve learned while establishing the protocols you find here.

Experimental Notes

Enzyme Protocols

Modifiable Protocol Template (for Microplate Enzyme Assay)

 

Enzyeme Protocols Flowsheet