Natural Resource Ecology Laboratory

International Workshop on Environmental Proteomics

Type: International Workshop
Date: January 19-22, 2010
Location: Keystone Resort, Keystone, CO


Primary Workshop Goal

The goal of this workshop is to assess the current state of the field of environmental proteomics, identify challenges, and elucidate a roadmap to guide us in overcoming such challenges specific to the analysis of complex communities.

Anticipanted Workshop Outcomes

  • Proposal to EMSL for side-by-side comparison of soil protein extraction methods
  • Formation of new environmental proteomics society
  • Roadmap (not review) papers on strategies for overcoming sample preparation and bioinformatics challenges

The final workshop program can be downloaded here.

Workshop Presentations

We had twelve presentations (including three keynote talks) over the course of the workshop. The slides from the presentations can be downloaded below and are for individual information only—please contact speakers for permission before using presentation contents in other applications.

Keynote Talks

Ken Reardon, Colorado State University
Environmental Proteomics: Potential, Challenges and Accomplishments
Martin von Bergen, Helmholtz Centre for Environmental Research
SIP goes Proteomics - Elucidation of structure and function of microbial communities
Ryan Mueller, University of California, Berkeley
Community proteomics reveals ecological relationships in a model microbial consortium

Session 1 - Overcoming Technical Challenges

Jessica Prenni, Colorado State University
Inter-lab Soil Protein Extraction Study
Nate Lawrence, Pressure Biosciences
Pressure BioSciences: Applying High Hydrostatic Pressure to Environmental Proteomics
Jeffery Werner, Cornell University
Targeted peptide quantification of Dehalococcoides proteins in a mixed culture during dehalorespiration
Ruth Richardson, Cornell University
Metaproteome changes during chloroethene-bioremediation and nutrient-starvation in enrichment cultures assayed using iTRAQ
Angela Norbeck, Pacific Northwest National Lab
Metaproteomics Data Analysis: An Interactive Visual Approach

Session 2 - From the Lab to the Field: Scaling up to Complex Natural Systems

Janet Jansson, Lawrence Berkeley National Lab
Soil metagenome projects as scaffolds for soil metaproteomics
Kathrin Riedel, University of Zurich
Metaproteomics to investigate the impact of sampling-site biogeochemistry on structure and functionality of leaf-litter degrading microbial communities
Robert Morris, University of Washington
Comparative Metaproteomics of Marine Bacteria and Archaea
Nathan VerBerkmoes, Oak Ridge National Lab
Gaining insight into bioremediation at the Rifle field site through proteogenomics techniques

Video Recordings of Select Presentations

Janet Jansson

Nate Lawrence

Robert Morris

Ryan Mueller

Angela Norbeck

Jessica Prenni

Kathrin Riedel

Nathan VerBerkmoes

Jeffery Werner

 


Funding Sources

Thanks to the Enzymes in the Environment RCN (NSF), Department of Energy, Pressure Biosciences, and Promega for providing funding for this workshop.