Phytase Hydrolysis of P-nitrophenyl Phosphate compounds
Phytase Hydrolysis of P-nitrophenyl Phosphate compounds
on 08/27/2009 10:41 by Senwo
In an in-vitro study, we showed that commercially available phytases from wheat and Aspergillus ficuum, hydrolyzed numerous p-nitrophenyl phosphate compounds [p-nitrophenyl phosphate disodium hexahydrate (>97%) (PNP), p-nitrophenyl phosphate di (2-amino-2-ethyl-1, 3 propanediol) (PNP2A2E), P-nitrophenyl phosphate bis (cyclohexylammonium) (PNPBC), Bis p-nitrophenyl phosphate sodium (Bis-PNP). This finding is amazing and lets us to believe that any result obtained for soil phosphatase activities assays include phytase activities. References: Tazisong, I.A., Z.N. Senwo, R.W. Taylor, and Z. He. 2008. Hydrolysis of organic phosphates by commercially available phytases: Biocatalytic potentials and effects of ions on their enzymatic activities. J. Food Agricul. Environ.6:500-505.
Thanks, Senwo